At the moment we are using a protocol with 24h formalin fixation and 24h decalcification but the achieved rna quality after laser capture microdissection is poor and also immunofluorescence. A laser capture microdissection protocol that yields high quality rna from fresh frozen mouse bones. Laser capture microdissection under a microscope using a software interface a tissue section typically 5 50 micrometres thick is viewed and individual cells or clusters of cells are identified either manually or in semi automated or more fully automated ways allowing the imaging and then automatic selection of targets for isolation.
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